Biological process  Cell communication ; Signal transduction  Molecular function  Receptor binding   Protein   tumor necrosis factor  Gene symbol   TNF    Cellular component           Component Extracellular Plasma membrane         Experimental evidence      Total number of studies : 22       Peptide sequence Method Platform  Sample  Reference Chemiluminescence assay - Serum Uzar et al. 2011 Chemiluminescent immunometric assay - Plasma Schmitt-Grohe et al. 2005 ELISA - Serum Alexandrakis et al. 2003 ELISA - Serum Tziakas et al. 2004 ELISA - Plasma Wong et al. 2005 ELISA - Serum Bruha et al. 2006 ELISA - Plasma Martin et al. 2007 ELISA - serum Davies et al. 2007 ELISA - Serum Higashimoto et al. 2008 ELISA - Serum Voros et al. 2011 ELISA - Serum Korczowska et al. 2011 Immunoassay - Plasma Straczkowski et al. 2002 Immunoassay - Plasma Haab et al. 2005 Immunoassay - Serum Antonelli et al. 2011 Multiplex beadbased Luminex technology - Plasma Tefferi et al. 2011 Multiplex immunobead assay - Serum Yurkovetsky et al. 2007 Multiple reaction monitoring QSTAR Pulsar Plasma Anderson et al. 2004 Mass spectrometry Plasma Liu et al. 2007 Mass spectrometry Fourier transform ion cyclotron resonance (FT_ICR) Plasma Qian et al. 2005 Mass spectrometry LTQ linear ion trap Plasma Jin et al. 2005 Mass spectrometry LTQ linear ion trap Plasma Tu et al. 2005 Mass spectrometry 4800 Proteomics Analyzer Plasma Bouwman et al. 2011          Multiple reaction monitoring data           Peptide sequence MH+ Sample Number of studies Reference DLSLISPLAQAVR  1381.8  Plasma  1  Anderson et al. 2004 DLSLISPLAQAVR  1387.8  Plasma  1  Anderson et al. 2004           Protein concentration             Protein concentration Method Sample Reference 5.9  pg/ml -  Plasma Polanski et al. 2007 8.3  pg/ml -  Plasma Anderson et al. 2004 37  pg/ml Immunoassay  Plasma Haab et al. 2005 0.0-4.9  pg/ml ELISA  Serum Korczowska et al. 2011 0.0-81.3  pg/ml ELISA  serum Davies et al. 2007 0.7-3.2  pg/ml Immunoassay  Serum Antonelli et al. 2011 0-10.9  pg/ml ELISA  Serum Bruha et al. 2006 0-15  pg/ml Multiplex beadbased Luminex technology  Plasma Tefferi et al. 2011 0-3.85  ng/ml Multiplex immunobead assay  Serum Yurkovetsky et al. 2007 0-67.6  pg/ml ELISA  Plasma Martin et al. 2007 1.32+/-0.39  pg/ml ELISA  Serum Alexandrakis et al. 2003 13.7+/-2.7  pg/ml ELISA  Plasma Wong et al. 2005 15.4+/-0.7  pg/ml Chemiluminescence assay  Serum Uzar et al. 2011 17.08-30.43  pg/ml ELISA  Serum Tziakas et al. 2004 4.07+/-0.24  pg/ml ELISA  Serum Voros et al. 2011 5.30+/-0.31  pg/ml ELISA  Serum Higashimoto et al. 2008 5.93+/-0.84  pg/ml Immunoassay  Plasma Straczkowski et al. 2002 8-75  pg/ml Chemiluminescent immunometric assay  Plasma Schmitt-Grohe et al. 2005           Extracellular vesicle proteins             Sample Isolation  Experimental method Vesiclepedia accession Reference Data not present          Architecture           Domains Motifs TM   35 - 57 TNF   88 - 233          External links           HPRD UniProt NetPath  01855  P01375  NetPath_M7124             Protein sequence  NP_000585.2               MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL