Biological process  Cell growth and/or maintenance  Molecular function  Extracellular matrix structural constituent   Protein   thrombospondin 1  Gene symbol   THBS1    Cellular component           Component           Experimental evidence      Total number of studies : 18       Peptide sequence Method Platform  Sample  Reference Mass spectrometry LTQ Plasma Looze et al. 2008 Radioimuunoassay - Plasma Hayden et al. 2000 Multiple reaction monitoring TSQ Vantage Plasma Huttenhain et al. 2012 Multiple reaction monitoring 6490 QQQ Plasma Domanski et al. 2012 Mass spectrometry LCQ DecaXP Serum Tirumalai et al. 2003 Mass spectrometry Plasma Liu et al. 2007 Mass spectrometry LTQ Orbitrap Serum Zeng et al. 2011 Mass spectrometry Fourier transform ion cyclotron resonance (FT_ICR) Plasma Qian et al. 2010 Mass spectrometry LTQ Orbitrap XL Plasma Panchaud et al. 2009 Mass spectrometry Fourier transform ion cyclotron resonance (FT_ICR) Plasma Qian et al. 2005 Mass spectrometry LTQ linear ion trap Serum He et al. 2005 Mass spectrometry IMS-TOF Plasma Valentine et al. 2006 Mass spectrometry LTQ FT Serum Sennels et al. 2007 Mass spectrometry LTQ FT Plasma Prentice et al. 2010 Mass spectrometry LTQ Plasma Tu et al. 2010 Mass spectrometry LCQ Plasma Ning et al. 2010 Mass spectrometry LTQ Orbitrap XL Serum Sinclair et al. 2011 Mass spectrometry - Plasma Farrah et al. 2011          Multiple reaction monitoring data           Peptide sequence MH+ Sample Number of studies Reference FVFGTTPEDILR  1394.7  Plasma  1  Huttenhain et al. 2012 GPDPSSPAFR  1036.5  Plasma  1  Domanski et al. 2012 GPDPSSPAFR  1030.2  Plasma  1  Domanski et al. 2012 GTLLALER  878.5  Plasma  1  Domanski et al. 2012 GTLLALER  872.5  Plasma  1  Domanski et al. 2012 GPDPSSPAFR  1030.4  Plasma  1  Domanski et al. 2012           Protein concentration             Protein concentration Method Sample Reference 200  ng/ml -  Plasma Anderson et al. 2004 210  ng/ml -  Plasma Polanski et al. 2007 510  ng/ml Spectral counting  Plasma Farrah et al. 2011 142-315  ng/ml Radioimuunoassay  Plasma Hayden et al. 2000           Extracellular vesicle proteins             Sample Isolation  Experimental method Vesiclepedia accession Reference Plasma - Normal  Filtration|Size exclusion|Sucrose density gradient  Mass spectrometry [LTQ]|Western blotting|Immunoelectron Microscopy  Vesiclepedia_7057 Looze et al. 2008           Architecture           Domains Motifs EGF   649 - 690 EGFCA   542 - 587 EGFCA   588 - 645 TSP1   382 - 429 TSP1   438 - 490 TSPN   24 - 221 VWC   318 - 372 CC   287 - 314 SP   1 - 19          External links           HPRD UniProt NetPath  01765  P07996  NetPath_M7057             Protein sequence  NP_003237.2               MGLAWGLGVL FLMHVCGTNR IPESGGDNSV FDIFELTGAA RKGSGRRLVK GPDPSSPAFR IEDANLIPPV PDDKFQDLVD AVRAEKGFLL LASLRQMKKT RGTLLALERK DHSGQVFSVV SNGKAGTLDL SLTVQGKQHV VSVEEALLAT GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSVFTRD LASIARLRIA KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVLL TLDNNVVNGS SPAIRTNYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK VTEENKELAN ELRRPPLCYH NGVQYRNNEE WTVDSCTECH CQNSVTICKK VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSTSCGNGIQ QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT CGDGVITRIR LCNSPSPQMN GKPCEGEARE TKACKKDACP INGGWGPWSP WDICSVTCGG GVQKRSRLCN NPTPQFGGKD CVGDVTENQI CNKQDCPIDG CLSNPCFAGV KCTSYPDGSW KCGACPPGYS GNGIQCTDVD ECKEVPDACF NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GQGVEHATAN KQVCKPRNPC TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN ENLVCVANAT YHCKKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH NPDQLDSDSD RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN DGIPDDKDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDSVP DIDDICPENV DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTPGQV RTLWHDPRHI GWKDFTAYRW RLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL FVFSQEMVFF SDLKYECRDP